Molecular experiment:
Real-time quantitative PCR, real-time quantitative RT-PCR, DNA extraction, RNA extraction, reverse transcription, protein extraction, Western-blot protein detection, ELISA detection,
CRISPR/Cas9: Construction of gRNA vectors;
CRISPR/Cas9: Construction of gene knockout cell lines;
CRISPR/Cas9: Construction of gene knockin cell lines.