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common problem

What are the advantages of dogs as biomedical animal models? What are the similarities between dogs and humans in immunology, genomics and physiology?

Beagles are gentle, cooperative and easy to operate. Dogs are the earliest animals domesticated by human beings. In the process of domestication, their living environment and eating habits tend to be basically the same with human beings. Therefore, there is a significant convergent evolution between domestic dogs and humans in the anatomical structure and physiological functions of digestion and metabolism, nervous system. Canine's social cognitive and emotional development has reached the level of human infants. In addition, there are about 450 kinds of spontaneous genetic diseases in dogs, which are very similar to human genetic diseases due to their similarity in function, metabolism and structure.
What are the behavioral paradigms of dogs？What behavioral instruments do you have?

Behavioral paradigm experiments of dogs: dog-dog three-box experiment, dog-dog single-box experiment, dog cognition experiment, dog help-seeking experiment, dog-human cage social experiment, dog treadmill experiment, dog climbing stairs experiment, dog sleep and activity analysis experiment, etc.

Behavioral instruments: dog sleep and activity analyzer, three-box track analysis, dog treadmill, etc.
What is the normal construction method for gene editing dogs? What is the period of the experiment? Delivery standards?

Gene editing dogs can be constructed by cytoplasmic injection and somatic cloning. The normal test cycle is 1 year (including vector construction, cell screening, clone construction, animal reproduction, etc.).

Delivery standard (limited to 1 year): Deliverable experimental animals are 2 F0 generation gene editing dogs (about 1 month old) after gene editing verification (report can be attached). If the customer needs a dog older than 1 month, the delivery time shall be postponed (signing a foster care contract).
What are the advantages and disadvantages of using somatic cloning method to build gene editing dogs?

Gene editing dogs can be constructed by cytoplasmic injection and somatic cloning. Compared with cytoplasmic injection, somatic cloning can obtain more consistent genotypes and more stable heredity, but the cost is higher than cytoplasmic injection.
How long does it take to obtain homozygous beagles by conventional gene editing method and somatic cloning respectively?

Homozygous beagles usually require two generations of breeding (F2 generations) by cytoplasmic injection and one year or two generations of breeding by somatic cloning.
How many F0 gene editing dogs can be obtained? Is it possible to ensure the gender of the dog? How to deal with the subsequent failure of reproduction of F0 generation?

Generally, 2 F0 generation gene editing dogs can be obtained at one time.

The gender of constructed gene editing dogs cannot be selected according to cytoplasmic injection, but can be selected by somatic cloning.

According to the actual situation, if F0 generation cannot be bred due to the influence of selected genes, the company will not bear relevant responsibilities (according to the contract); If breeding is not possible due to the absence of reproductive line chimerism after gene editing, another procedure can be negotiated.