Products services Q&A

common problem

Can the company construct gene editing dogs by transgenic, gene knockout, gene insertion or point mutation? Is there other technique can be used?

The company can construct gene editing dogs by transgenic, gene knockout, gene insertion or point mutation. No other techniques are used.
Is the company using CAS9 technology for gene editing model dogs? How to solve the miss problem about CAS9?

Gene knockout and insertion are realized based on CRISPR/Cas9 technology. The products we delivered to customers have passed off-target analysis test.
What's the difference between cytoplasmic injection and somatic cloning technology? What's the difficulty in cytoplasmic injection?

The F0 generation gene editing animals obtained by cytoplasmic injection are chimeric animals, whose genotypes can only be tested after the birth of the animals, so gender cannot be screened, and there is a risk of non-reproductive chimerism. The genotypes of F0 generation obtained by somatic clone were the same as those of donor cells, which could be selected for gender and inherited stably. The technicians using cytoplasmic injection must have high skills, and the gene editing dogs obtained are mostly chimeras.
What kind of gene editing animals are currently available by the company?

At present, standard experimental animals such as dogs, pigs, rabbits and mice can be constructed for gene editing.
What kind of routine experiments can the company do now?

At present, the company can do routine experiments including routine surgery model, tumor model, diabetes model and so on.
What are sinogenetic's advantages as a CRO enterprise?

Relying on the cloning and gene editing technology platforms of the parent company, Sinogenetic can provide CRO services for both gene editing animals and conventional experimental animals. We have behavioral platform, interventional operating room, ophthalmology platform, electrophysiology platform etc. to meet the various experimental requirements of customers, and can evaluate the safety and effectiveness of medical devices on dogs.