Hemorrhagic shock model of beagle

Hemorrhagic shock model of beagle

Hemorrhagic shock belongs to hypovolemic shock, which is more common in acute and rapid blood loss, which sharply reduces the effective circulation of the body and causes insufficiency of blood perfusion in the tissues of the whole body, and damages the functions of multiple organs, resulting in tissue ischemia and hypoxia, metabolic disorders and neurological dysfunction. Its condition is dangerous, changes quickly, easily leads to the death of the patient. Once hemorrhagic shock occurs, immediate treatment should be carried out to prevent adverse consequences.

Experimental animals: Male beagle, 10 ~ 12 months old, weight 10 ~ 12 kg.

Model making method:

1.Endotracheal intubation, 1.0% isoflurane was used to maintain anesthesia, and anesthesia machine was used to control breathing. The skin and muscles of the neck were cut open, the carotid artery and the vagus nerve were separated, the carotid artery and the jugular vein were found, the proximal and distal ends of the carotid artery were clamped with a vascular clamp, and the distal ends were ligated with no.4 nonabsorptive surgical suture, and the distal end of the carotid artery clamp was released. The corresponding catheter was inserted, the carotid catheter was used to collect arterial blood samples and measure blood pressure during surgery, and the jugular catheter was used for bloodletting. Blood is quickly drained from the carotid catheter to an arterial systolic pressure of about 50mmHg, and the released blood is recycled into plastic blood bags containing anticoagulants to maintain blood pressure through continued bleeding or transfusion.

2.Arterial blood samples were collected for blood gas analysis after 30 min bloodletting.

3.When arterial lactic acid and arterial alkine surplus reached the standard, venous blood samples were collected to detect venous oxygen saturation. (The criteria for model success were arterial lactic acid 9-11mmol /L, arterial alkine residue < -12 mmol/L, and venous oxygen saturation < 60%.)

Model identification:

Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MAP), heart rate, temperature, capillary refill time (CRT), pulse oxygen saturation (SpO2), arterial lactic acid (Lac), arterial alkine residue (BE), venous oxygen saturation (SvO2), arterial pH, arterial partial pressure of carbon dioxide (PCO2), arterial partial pressure of oxygen (PO2), hemoglobin content (HGB), hematocrit (HCT), blood discharge and transfusion volume during shock.

Application:

As the accurate evaluation of the efficacy of antishock drugs adopts multiple indicators as the criteria for successful modeling, the model more objectively and accurately simulates the state of hemorrhagic shock in clinical patients, providing a reliable platform for us to evaluate blood products and blood substitutes in the next step. The experimental model is very close to the resuscitation measures and clinical treatment, which can be used to study the organ function damage after shock, i.e. the effect of fluid resuscitation.