Parkinson model

Parkinson model

Parkinson's disease is a neurodegenerative disease that usually affects older adults. The main manifestations are slow movement, tremors in hands, feet or other parts of the body, and the body loses its softness and becomes stiff.

Experimental animals: 1 to 2 years old, weight 13-15 kg, all male beagles.

Model making method:

1.Place an indwelling needle from the dog's forelimb and bind and fix it with medical tape.

2.Slowly push in the freshly configured MPTP solution (0.2-0.4mg/kg, Sigma Company).

3.In the same way, from the first injection, the dogs were injected with the same amount of MPTP solution every other day for 5 consecutive times.

4.After the last injection, the experimental dogs were provided with orange juice containing levodopa three times a day at regular intervals. 

Model identification:

1.Behavioral evaluation Scale for Parkinson's disease was used. Behavioral scores and videos were performed on experimental monkeys at regular intervals every day before the given of levodopa.

2.Brain histopathological examination: 3 months after modeling, the dog was anesthetized and fixed by intracardiac perfusion with normal saline and paraformaldehyde. The experimental monkey brain was completely removed and dehydrated by hypertonic sucrose water. The thalamus and brain stem were sectionally sliced for HE and TH staining.

Application: To verify the efficacy of drugs in treating Parkinson's disease