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Canine model of osteoporosis
Osteoporosis is the most common bone disease. It is a systemic bone disease characterized by low bone mass, damage of bone tissue microstructure, increased bone fragility and easy fracture. Osteoporosis can occur at any age, but is more common in postmenopausal women and older men.
Experimental animals: female dogs aged 5-6 years with an average weight of about 16kg.
Model making method:
After anesthesia, a sterile abdominal midline incision was made to separate the milky cellulite surrounding the ovaries. The fallopian tubes were ligated with thread and the yellow ovaries were removed, while the other ovary was removed. The incisions were sutured layer by layer and antibiotics and analgesics were injected.
Model identification:
1.Blood biochemical examination: bone metabolism (alkaline phosphatase, phosphorus, magnesium, serum uric acid, total calcium, total acid phosphatase, non-prostaglandate phosphatase, prostaglandate phosphatase) and estradiol before and after surgery.
2.After 12 weeks, the animals were killed by intravenous injection of potassium chloride injection. Bone samples were taken from femur, tibia and fibula, ulnar and radial bones, and lumbar vertebrae for BMD measurements, as well as trabecular and cortical changes.
Application: it can be used to study postmenopausal osteoporosis and artificial joint replacement model through femoral head surgery.
