Canine Model of femoral head necrosis 

Canine Model of femoral head necrosis 

Femoral head necrosis is a common disease of the hip joint, in which the blood supply of the femoral head is interrupted or damaged, resulting in the death and subsequent repair of bone cells and bone marrow components, then further resulting in structural changes of the femoral head, femoral head collapse, and joint dysfunction.

Experimental animals: beagles over 6 months old, weighing about 10 kg.

Model making method:

1.The right femur of experimental Beagle dogs was modeled, and the left side was used as the control.

2.Take the posterolateral incision of the hip joint, about 5cm long, cut the skin and fascia in turn, and separate the muscle to the joint capsule.

3.Open the joint capsule to expose the femoral head.

4.The femoral head is filled with dry gauze to protect the tissue around the femoral head.

5.An iron funnel was used to cover the femoral head, and 100 ~ 150 mL liquid nitrogen was poured into the funnel and kept frozen for 3 min.

6.Closure: the femoral head was rewarmed with 37 ℃ saline, and the incision was sutured layer by layer. First, the muscle layer and fascia layer were closed with a round needle with 4-0 line, and then the skin layer was closed with Angle needle. Iodophor is used to disinfect the wound and a sterile dressing is applied. Intramuscular injection of ceftriaxone sodium (5mg/kg) for anti-infection, intramuscular injection of 0.5ml tongliding, put into the nursing cage for observation.

Model identification:

1.One month after modeling, 6 Beagle dogs were randomly selected for MRI scanning and pathological examination.

2.MRI scanning and histopathological examination were performed on the remaining 6 dogs 2 months after modeling.

Application: Etiology research, treatment research.